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| Study on permeabilities of Salidroside via rat mesentery mucosa by using Ussing Chamber |
| Hits 2135 Download times 1825 Received:April 25, 2016 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2016.11.14 |
| Key Words
salidroside;Ussing Chamber;P-gp;tight junction;verapamil;SDS |
| Author Name | Affiliation | E-mail | | GU Xing | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YAO Shao-zi | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | PI Jia-xin | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Bo-ying | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HAN Xu | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Zhi-dong | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | lonerliuzd@163.com |
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| Abstract
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| [Objective] To investigate the permeability of salidroside in mesentery mucosa of rat ex vivo.[Methods] The Ussing Chamber was employed to investigate the ex vivo permeability in rat mesentery mucosa of salidroside with different concentrations or with the addition of verapamil and SDS.[Results] It was found that the Flux of salidroside was increased with the increasing concentration, and there was no difference(P<0.05) in the apparent permeability coefficient (Papp) in different mesentery segments of rat. The Papp of salidroside got significantly increased in ileum and colon (P<0.01 or P<0.05), and reached to 2.76-fold compared with the control in ileum, when a certain amount of SDS was added in the donator side.[Conclusion] Salidroside could be slightly absorbed in all mesentery segments of rat with a passive concentration-dependent transportation. There were no effects of verapamil on permeation of salidroside, and SDS could significantly increase salidroside permeation in ileum and colon of rat. |
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