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| Curcumin regulation of human lens epithelail cell proliferation through Wnt/β-catenin signaling pathway |
| Hits 1760 Download times 1705 Received:August 26, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.12.11 |
| Key Words
curcumin;Wnt3a;lens epithelial cell;Wnt/β-catenin signaling pathway;cell proliferation;posterior capsular opacification |
| Author Name | Affiliation | | BAO Xiu-li | Department of Ophthalmology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China | | LIU Ting-ting | Department of Ophthalmology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China | | ZHANG Hai-tao | Department of Ophthalmology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China | | ZHANG Li-min | Department of Ophthalmology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China |
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| Abstract
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| [Objective] To investigate the effects of curcumin on proliferation of human lens epithelial cells (LECs) and its mechanism and to provide a new gene target in the prevention and treatment of PCO.[Methods] The research objects were the human LECs SRA 01/04.The experiment was divided into three groups:Wnt3a group,curcumin group and control group.In Wnt3a group,human Wnt3a cDNA expressing vector targeted human LECs was constructed to PCO model.In curcumin group,curcumin of 20 μmol/L was added after 48 hs of transfection.pcDNA3-HA expression vector was used as the control group.The expression of Wnt3a was identified by Western blot assay after transfected.The growth and proliferation of SRA 01/04 cells were detected by CCK-8 test.The expression and localization of proliferating cell nuclear antigen (PCNA) were analyzed by immunocytochemistry for the exploration of the mechanism of curcumin to proliferation of LECs.The expressions of cyclin D1and c-Myc in the cells were detected by Western blot assay.β-Catenin expression was localized using immunofluorescence assay.[Results] The expression of Wnt3a was verified in the Wnt3a transfected group compared with the control group.CCK-8 test indicated that the cell proliferating rate was significantly difference between the curcumin group and Wnt3a group(t=2.782,P=0.049).The positive expression rate of PCNA protein in SRA 01/04 was 23.6%±4.0% in the curcumin group and 43.4%±5.4% in the Wnt3a group with a significant difference between them (t=2.951,P=0.018).After 48 hours of treatment with curcumin,the immunofluorescence was stronger in cell nucleus,and the expressions of cyclin D1 and c-Myc proteins were elevated in SRA 01/04 cells in wnt3a group,but he immunofluorescence was stronger in cytoplasm,and the expressions of cyclin D1 and c-Myc proteins were less in SRA 01/04 cells in curcumin group and controls.[Conclusion] Curcumin depressed the Wnt/β-catenin signaling pathway and downregulates the expression of a subset of target genes,including cyclin D1 and c-Myc,which plays an important role in inhibiting the proliferation of human LECs. |
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