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| Simultaneous determination of six chemical components for quality control of Semen Descurainiae by HPLC-UV |
| Hits 1913 Download times 1554 Received:April 26, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.12.12 |
| Key Words
Semen Descurainiae;HPLC-UV;content determination |
| Author Name | Affiliation | E-mail | | JIANG Yan | Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YIN Xue-yan | Traditional Chinese Medicine College, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Jin | Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Hui | Traditional Chinese Medicine College, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | DOU Zhi-ying | Traditional Chinese Medicine College, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yan-xu | Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tcmcyx@126.com |
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| Abstract
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| [Objective] An HPLC-UV method was established for simultaneous determination of the content of six components (quercetin-3-O-β-D-glucise-7-O-β-D-gentiobiosiden,quercetin-3-O-β-D-glucoside,isorhamnetin-3-O-β-D-glucoside,quercetin,kaempferol and isorhamnetin) in Semen Descurainiae in order to provide a theoretical basis for its quality assessment.[Methods] The analysis was performed on Agilent eclipse plus C18 column (4.6 mm×100 mm and 1.8 μm).Acetonitrile -0.1% phosphoric acid water solution was used as mobile phase at flow rate of 0.3 mL/min.The column temperature was 30℃ and the detection wavelength was set at 265 nm.[Results] Six components were well separated with good linear relationship.The recoveries were 99.1%~103.0%.[Conclusion] The accurate and reliable HPLC-UV method was established and could be used for quality evaluation of Semen Descurainiae. |
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