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| Analysis of absorbed components in rat plasma after oral administration of Lianhua Qingwen capsules by UPLC-Q-TOF-MS |
| Hits 2644 Download times 1787 Received:July 01, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2016.12.13 |
| Key Words
Lianhua Qingwen capsule;absorbed components in plasma;UPLC-Q-TOF-MS |
| Author Name | Affiliation | E-mail | | YU Hua | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | JIA Wei-na | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | LIU Jin-ping | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | ZHU Yan | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | WANG Chun-hua | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | pharmwch@126.com |
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| Abstract
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| [Objective] A UPLC-Q-TOF-MS method was developed for the analysis of absorbed components in SD rat plasma after oral administration of Lianhua Qingwen capsules (LQC) for the first time.[Methods] The blood samples were taken from the posterior orbital venous plexus of rats and methanol was used for the protein precipitation.The separation of components was achieved on a C18 column by gradient elution with methanol -0.1% formic acid water solution as mobile phase.Comparative analysis of blank plasma,mixed reference standards and plasma with LQC was performed by UPLC-Q-TOF-MS in negative ion mode.[Results] The results suggested that 22 prototype compounds were identified,including flavonoid,anthraquinone,phenylpropanoid and iridoid.These compounds were identified and confirmed by comparing their retention times,mass spectrometry fragmentation characteristics with that of references as well as literatures.[Conclusion] The study of the absorbed components of LQC in plasma provides an experimental basis for revealing the effective components and its pharmacokinetics study. |
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