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| Experimental study of Xinfukang pills on microRNA-1 in cardiac tissue of pressure overloaded rats |
| Hits 2195 Download times 1906 Received:September 13, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.01.12 |
| Key Words
Xinfukang pill;microRNA-1;pressure overload;myocardial hypertrophy |
| Author Name | Affiliation | | LIANG Yong | Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Intensive Care Unit of Tianjin Beichen District Chinese Medicine Hospital, Tianjin 300400, China | | GUO Peng | Pharmacy Department of Tianjin Children's Hospital, Tianjin 300134, China | | CHEN Zuo | Intensive Care Unit of Tianjin Beichen District Chinese Medicine Hospital, Tianjin 300400, China | | LI Yan-ling | Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Intensive Care Unit of Tianjin Beichen District Chinese Medicine Hospital, Tianjin 300400, China | | LIU Yong-yu | Intensive Care Unit of Tianjin Beichen District Chinese Medicine Hospital, Tianjin 300400, China | | SUN Ling | Intensive Care Unit of Tianjin Beichen District Chinese Medicine Hospital, Tianjin 300400, China | | CAO Shu-hua | Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | GAO Ke-jian | Intensive Care Unit of Tianjin Beichen District Chinese Medicine Hospital, Tianjin 300400, China |
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| Abstract
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| [Objective] To explore improving effect of Xinfukang pills on pressure overload-induced myocardial hypertrophy and ventricular remodeling in rats, the relationship between its pharmacology effect and the express of miR-1 and in cardiac tissue was evaluated.[Methods] The experiment adopted the method of narrowing and contraction the aorta in adult and healthy male Wistar rats to establish the model of animals with myocardial hypertrophy and ventricular remodeling. Four weeks after coarctation of abdominal aorta, the Wistar rats were randomly divided into model group (M group), Enalapril group(E group), Xinfukang pills low dose group(L group) and Xinfukang pills high dose group (H group). Sham surgery group did not ligate the abdominal aorta, other treatments were same as others. 8 and 12 weeks after surgery, myocardial tissue collagen volume fraction was evaluated by Masson dyeing. The express of SERCA2 and Fibullin-2 in cardiac tissue were detected by immunohistochemistry. The express of miR-1 was also detected by Real time PCR. [Results] The 8 and 12 weeks after surgery, compared with group M, the myocardial fibrosis area of group E and H were reduced significantly (P<0.01), and the express of Fibullin-2 in the cardiac tissue of group H was decreased significantly (P<0.01). The express of SERCA2 in the cardiac tissue of group H was increased significantly (P<0.01). The express of miR-1 in the cardiac tissue of group H increased significantly (P<0.01). After 8 weeks, the express of Fibullin-2 in the cardiac tissue of group L was different with group M (P<0.05).[Conclusion] Xinfukang pills can increase the express of miR-1 and regulating the target protein Fibullin-2, therefore it may inhibit apoptosis of myocardial fibrosis, and plays an important role in repressing cardiac hypertrophy and ventricular remodeling process. |
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