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| Experimental research on the influence of cinnamic aldehyde on the proliferation, apoptosis and cell cycle of fibroblast-like synovial cells |
| Hits 2500 Download times 2200 Received:August 21, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.01.13 |
| Key Words
osteoarthritis;cinnamic aldehyde;fibroblast-like synovial cell;proliferation;flow cytometry |
| Author Name | Affiliation | E-mail | | WANG Huan | Beijing University of Chinese Medicine, Beijing 100029, China | | | WANG Qing-fu | The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China | qingpu-wang@sohu.com | | YANG Li-li | Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHANG Dong | Beijing University of Chinese Medicine, Beijing 100029, China | | | WANG Wei-li | Beijing University of Chinese Medicine, Beijing 100029, China | | | DING Hao-bin | Beijing University of Chinese Medicine, Beijing 100029, China | | | GUO Yu-ru | Beijing University of Chinese Medicine, Beijing 100029, China | | | DU Wang-yang | Beijing University of Chinese Medicine, Beijing 100029, China | | | XU Ming-kang | Beijing University of Chinese Medicine, Beijing 100029, China | | | GAN Wen | Beijing University of Chinese Medicine, Beijing 100029, China | |
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| Abstract
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| [Objective] To investigate the influence of different concentrations of cinnamic aldehyde (CA) on the proliferation, apoptosis and cell cycle of fibroblast-like synovial cells(FLS), and then explore the regulatory mechanism of CA to the synovial inflammation in osteoarthritis.[Methods] Different concentrations of CA were intervened on LPS-induced FLS respectively, and the proliferation, apoptosis and cell cycle were measured through Methylthiazolyldiphenyl-tetrazolium bromide assay (MTT) and flow cytometry(FCM).[Results] Low concentration of CA could promote the growth of FLS mildly, while high concentration of CA could significantly reduce the survival rate (P<0.05) in concentration-dependent manner. LPS could reduce the apoptosis rate of FLS, shorten the G1 phase and extend the S phase, it meant that the inflammatory model was successfully established. Compared with the control group, both high and low concentration of CA groups could obviously increase the ratio of cells in the areas of Q2 and Q4 (P<0.05), and extend the G1 phase and S phase (P<0.05) to induct the apoptosis and inhibit the proliferation of FLS, especially the effect of high concentration of CA group was more significant.[Conclusion] Different concentrations of CA has certain inhibition for the growth of FLS, and the high concentration of CA group has stronger inhibitory effect, which may be the potential mechanism of CA's anti-inflammatory effects. |
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