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| Study on a rapid extraction method and content determination method of total cardiac glycosides from leaves of Thevetia peruviana |
| Hits 2265 Download times 2094 Received:August 25, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.01.14 |
| Key Words
Thevetia peruviana;cardiac glycoside;extraction process;1H-NMR |
| Author Name | Affiliation | E-mail | | WEN Shi-yuan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | CHEN Yan-yan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | LI Xiao-nan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Research and Development Center of Traditional Chinese Medicine Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China | | | WANG Yue-fei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIANG Miao-miao | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | miaomiaojiang@126.com |
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| Abstract
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| [Objective] To study rapid extraction method and content determination method of total cardiac glycosides from leaves of Thevetia peruviana.[Methods] Using 1H-NMR to content determination of cardiac glycosides, 4 kinds of extraction process, the methanol soaking, 70% ethanol heating refluxing, 70% ethanol soaking, 95% ethanol gentle soaking, were screened.[Results] The 95% ethanol gentle soaking method extracted the highest amount of cardiac glycosides.[Conclusion] The extraction process of 95% ethanol gentle soaking is a better fast extraction method for extracting total cardiac glycoside from leaves of Thevetia peruviana, and content determination of cardiac glycosides can be employed by the 1H-NMR. |
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