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| The preparation and preliminary evaluation of anti-tumor effect of Gambogic acid nanostructure lipid carrier |
| Hits 2409 Download times 2008 Received:May 07, 2016 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2017.01.15 |
| Key Words
gambogic acid;nanostructure lipid carrier;physical and chemical properties;anti-tumor effect |
| Author Name | Affiliation | E-mail | | HUANG Rui | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine Provice and Ministry Co-established State Key Laboratory Cultivation Base, Tianjn University of Traditional chinese Medicine, Tianjin 300193, China | | | GUO Pan | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine Provice and Ministry Co-established State Key Laboratory Cultivation Base, Tianjn University of Traditional chinese Medicine, Tianjin 300193, China | | | LIU Bo-ying | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine Provice and Ministry Co-established State Key Laboratory Cultivation Base, Tianjn University of Traditional chinese Medicine, Tianjin 300193, China | | | DING Miao-miao | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine Provice and Ministry Co-established State Key Laboratory Cultivation Base, Tianjn University of Traditional chinese Medicine, Tianjin 300193, China | | | ZHANG Bing | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine Provice and Ministry Co-established State Key Laboratory Cultivation Base, Tianjn University of Traditional chinese Medicine, Tianjin 300193, China | | | ZHANG Li-na | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine Provice and Ministry Co-established State Key Laboratory Cultivation Base, Tianjn University of Traditional chinese Medicine, Tianjin 300193, China | | | LIU Zhi-dong | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine Provice and Ministry Co-established State Key Laboratory Cultivation Base, Tianjn University of Traditional chinese Medicine, Tianjin 300193, China | lonerliuzd@163.com |
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| Abstract
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| [Objective] To prepare the nanostructure lipid carriers (NLC) incorporated gambogic acid (GA), investigate its physical and chemical properties and evaluate the anti-tumor effect preliminarily.[Methods] Emulsification-evaporation was used to prepare the nanoparticles. The particle size, Zeta potential, encapsulation efficiency were selected as the evaluation indexs to appraise the physical and chemical properties and process validation was implemented by using differential scanning calorimetry (DSC). The CCK-8 assay was used to measure human breast cancer cells MDA-MB-231 cell growth repression rate.[Results] The GA-NLC was about 20 nm in diameter. Zeta potential was -(5.86±0.64) mV. The encapsulation efficiency was (99.46±0.23)%. DSC results showed that GA exists in gambogic acid nanostructure lipid carrier in the form of amorphous.[Conclusion] The GA-NLC prepared by emulsification-evaporation was characterized of smaller particle size, uniform distribution and high encapsulation efficiency. Compared with GA solution, GA-NLC has a significant antitumor activity. |
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