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| The target-finding and mechanism of drug action researching of Kechuan Liuwei Mixture treating asthma of cold type based on cDNA microarrays technology |
| Hits 1957 Download times 1367 Received:September 20, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.02.06 |
| Key Words
Kechuan Liuwei Mixture;asthma of cold type;cDNA microarrays technology;target gene;mechanism of drug action |
| Author Name | Affiliation | | ZOU Lu | Preventive Treatment Center, Longhua Hospital Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China | | FANG Hong | Preventive Treatment Center, Longhua Hospital Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China | | WU Yin-gen | Preventive Treatment Center, Longhua Hospital Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China |
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| Abstract
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| [Objective] The aim of this study was to explore the target genes and mechanism of drug action of Kechuan Liuwei Mixture.[Methods] The 16 patients who were diagnosed with asthma of cold type were selected to do the gene chip screening. The 8 cases of them took Kechuan Liuwei Mixture three times a day, 20 mL per time, while the others took the placebo. Both groups were treated for one week. Peripheral blood samples were taken after one-week drug intervention. Total RNA was extracted from peripheral blood. Using gene expression times numerical 1.5 and -1.5 for threshold to determine the differential expression genes,then GO and pathway analysis were used to analyze the differential expression genes on function and classification.[Results] Screening and identification of differentially expressed genes, 233 1.5-fold DEGs were screened from the gene expression spectrum, while 106 genes were up-regulated and 127 genes were down-regulated. These differently expressed genes are involved in functions of inflammatory responses,immune response,Cytokine-cytokine receptor interaction,transforming growth factor and pathways of Cytokine-cytokine receptor interaction, Focal adhesion, Calcium signaling pathway, MAPK signaling pathway, ECM-receptor interaction of asthma.[Conclusion] Kechuan Liuwei Mixture could treat asthma of cold type through multi-channels and multi-targets by effecting genes that related to immune response, inflammatory reaction, airway remodeling and calcium channels. |
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