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| Effect of Survivin siRNA on human lung adenocarcinoma A549 cell proliferation and apoptosis mediated by drug-containing serum of Xiaoyan decoction |
| Hits 1619 Download times 1577 Received:October 09, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.02.12 |
| Key Words
Xiaoyan decoction;Survivin siRNA;A549;proliferation;apoptosis |
| Author Name | Affiliation | E-mail | | ZHANG Xin | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIA Ying-jie | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | 贾英杰,jiayingjie1616@sina.com | | YANG Pei-ying | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Yun-chao | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To analyze effect of Survivin siRNA on human lung adenocarcinoma A549 cell proliferation and apoptosis mediated by drug-containing serum of Xiaoyan decoction and probe into its mechanism of tumor suppression.[Methods] The siRNA targeting survivin gene sequence was designed by Invitrogen Company's online design system, and A549 cells were transfected. The growth inhibition of A549 cell by Xiaoyan decoction group and transfection group were detected by MTT method, the expression of Survivin protein of A549 cells was observed immunohistochemical method. Apoptosis of cells was detected by flow cytometry.[Results] MTT method showed that the effect of A549 cell growth was inhibited by the Xiaoyan Decoction and its function has certain time dependence. The inhibition rate of the transfection TCM group was significantly higher than that of the transfection control group and non transfection TCM group. Detected by immunohistochemical method, the inhibition rate of Survivin protein expression in transfected control group was significantly higher than that in non transfection TCM group, but significantly lower than transfection TCM group. Flow cytometry test results show that Xiaoyan Decoction and recombinant plasmid is given priority to inducing cell apoptosis in advanced stage, A549 cell apoptosis rate of transfection TCM group was significantly higher than other groups.[Conclusion] Xiaoyan Decoction can induce apoptosis of A549 cells. Combined the use of transfection targeting Survivin gene recombinant plasmid can enhance the effect of the apoptosis induction of A549 cells in vitro. |
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