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| Preliminary experimental study on the establishment and evaluation of mice model about coronary heart disease with phlegm and blood stasis syndrome |
| Hits 2317 Download times 1535 Received:November 01, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.02.13 |
| Key Words
coronary heart disease;phlegm and blood stasis syndrome;ApoE knockout mice;isoprenaline;model |
| Author Name | Affiliation | E-mail | | GAO Shan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YIN Jia | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | SONG Yan-qi | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YANG Kai-lun | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHOU Hao-bo | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | PAN Ye | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Lin | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | XU Yi-lan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YU Chun-quan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | ycq-4@163.com |
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| Abstract
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| [Objective] To establish and evaluation the method of mouse model about coronary heart disease with phlegm and blood stasis syndrome.[Methods] Mice were divided into blank group:10 C57BL/6J mice, fed ordinary feed 6 weeks; phlegm and blood stasis syndrome model group:10 ApoE knockout mice, fed high fat diet for 6 weeks, and isoprenaline by subcutaneous injection (10 mg/kg) for 7 d before the mice to death, 1 time a day, and blank group was given the same amount of saline solution. To observe the general condition of mice in the experiments, the ultrasonic detecting of small animals and serum lipid levels were detected at the 6th weekend, than the model were evaluated.[Results] Compared with the blank group, phlegm and blood stasis syndrome model group mice IVSd, IVSs, LVPWd, LVPWs were thickening, and there were significant(P<0.01), and heart presented characteristics of myocardial hypertrophy, and LVM significantly increased (P<0.05 or P<0.01). The serum TC, TG and LDL-C of mice increased significantly(P<0.05 or P<0.01).[Conclusion] ApoE knockout mice fed by high fat feed combined with isoprenaline induced can establish coronary heart disease with phlegm and blood stasis syndrome of mouse model. The model is stable, easy to operate, and has good repeatability. |
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