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| Preliminary study on the effect of total glycosides from Cornus Officinalis on treatment of acute immunologic liver injury induced by ConA in mice |
| Hits 1890 Download times 1388 Received:November 12, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.02.14 |
| Key Words
total glycosides from Cornus Officinalis;ConA;inflammatory factor;immunologic liver injury |
| Author Name | Affiliation | E-mail | | ZHAO Chen-xiang | Postgraduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Ya-min | Department of Hepatobiliary Surgery in First Central Hospital, Tianjin 300192, China | zhangyamin@medmail.com.cn | | LIU Hong-sheng | Key Laboratory of Emergency Medicine of Critical Disease of Healthful Ministry in First Central Hospital, Tianjin 300192, China | | | LIU Zi-rong | The First Central Clinical College, Tianjin Medical University, Tianjin 300192, China | |
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| Abstract
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| [Objective] To study the protective effect of total glycosides from Cornus Officinalis (TGCO) on immunological liver injury induced by concanavalin A(ConA) in mice.[Methods] The 60 C57BL mice were randomly divided into 6 groups:the normal group, the model group, positive control biphenyl group, TGCO high does group, TGCO middle dose group and TGCO low dose group (920, 460, 230 mg/kg), with 10 mice in each group. Except normal mice, all the other mice were injected ConA 15 mL/kg once intraperitoneally and the mice in the normal groups received an equal volume of saline. The 2 h later after the annimalmodel was created, normal group and model group were given normal saline(0.02 mL/g) by gavage, positive control group was given bifendate (100 mg/kg) by gavage. TGCO high does group, TGCO middle dose group and TGCO low dose group were given TGCO at the dosage of 920, 460 and 230 mg/kg respectively, once every six hours for five times. Two hours after final administration, the mice were sacrificed and weighted to compared liver index and spleen index and detect content of serum ALT and AST,TNF-α, IFN-γ, SOD, OCT, IL-1 and IL-6 in liver homogenate. Liver pathological changes were observed by HE staining.[Results] Both of TGCO and biphenyl remarkably decreased the increased live index and spleen index (P<0.05), improve the histopathological changes in liver and pathological grades of liver tissues and relieve the inflammatory reaction induced by ConA. TGCO effectively decreased the activities of ALT and AST in serum and the decreased contents of TNF-α,IFN-γ, IL-1, IL-6 and OCT in hepatic tissues, and increased levels of SOD in liver homogenate (P<0.05).[Conclusion] TGCO can obviously protect immunological injury liver a dose-dependent manner in the range of test doses, the mechanism possibly due to its function of anti-inflammatory, anti-oxidation, scavenging oxygen free radical and regulating immunity. |
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