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| Effect of Purendan on glucolipid metabolism and insulin resistance in ob/ob mice |
| Hits 2154 Download times 2196 Received:December 26, 2016 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2017.03.10 |
| Key Words
Purendan;obesity;lipid metabolism;insulin resistance;ob/ob mice |
| Author Name | Affiliation | E-mail | | ZHANG Yao-dan | Minzu University of China, Co-constructed Key laboratory of Chinese Traditional Medicine by State Ethnic Affairs Commission and Ministry of Education of the People's Republic of China, Beijing 100081, China | | | CHEN Shu | Minzu University of China, Co-constructed Key laboratory of Chinese Traditional Medicine by State Ethnic Affairs Commission and Ministry of Education of the People's Republic of China, Beijing 100081, China | | | SU Leng-gao-wa | Minzu University of China, Co-constructed Key laboratory of Chinese Traditional Medicine by State Ethnic Affairs Commission and Ministry of Education of the People's Republic of China, Beijing 100081, China | | | BAI Ying-hui | Minzu University of China, Co-constructed Key laboratory of Chinese Traditional Medicine by State Ethnic Affairs Commission and Ministry of Education of the People's Republic of China, Beijing 100081, China | | | YANG Kun-bao | Minzu University of China, Co-constructed Key laboratory of Chinese Traditional Medicine by State Ethnic Affairs Commission and Ministry of Education of the People's Republic of China, Beijing 100081, China | | | LU Bi-nan | Minzu University of China, Co-constructed Key laboratory of Chinese Traditional Medicine by State Ethnic Affairs Commission and Ministry of Education of the People's Republic of China, Beijing 100081, China | | | PANG Zong-ran | Minzu University of China, Co-constructed Key laboratory of Chinese Traditional Medicine by State Ethnic Affairs Commission and Ministry of Education of the People's Republic of China, Beijing 100081, China | zrpang@163.com |
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| Abstract
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| [Objective] To investigate effect of Purendan on glucolipid metabolism and insulin resistance in ob/ob mice, and provide experimental basis for clinical application of Chinese herbal compound Purendan which can regulate glucolipid metabolism.[Methods] The ob/ob mice were randomly divided into model group and Purendan group, which received the Purendan intervention for 15 weeks, with C57BL/6J mice as control group. The fasting body weight, visceral fat index, serum lipid level, fasting blood glucose (FPG), fasting serum insulin (FINS) were investigated. The level of insulin resistance and secretion was estimated by homeostasis model assessment of insulin resistance index (HOMA-IR) and insulin sensitivity index (ISI). The level of regulating blood sugar was observed by oral glucose tolerance test (OGTT). Histopathological changes of the adipose tissue were observed by HE staining.[Results] The fasting body weight and visceral fat index, the area under the OGTT curve, serum lipid level and HOMA-IR were significantly increased, and ISI was significantly decreased in the ob/ob mice of model group. The effect of Purendan on ob/ob mice fasting body weight, visceral fat index and glucose tolerance was not statistically significant. Purendan can significant reduce the levels of total cholesterol (TC) and low density lipoprotein (LDL) and HOMA-IR, and significant increase ISI. Purendan can also make the arrangement of epididymal fat cells in ob/ob mice was relatively regular.[Conclusion] After 15 weeks of observation, the ob/ob mice in model group were formed obesity, dyslipidemia, impaired glucose tolerance, insulin resistance, which means that the model of pre-diabetes was copied successfully. Purendan effectively improved glucose and lipid metabolism disorder, increased insulin sensitivity and decreased insulin resistance degree in ob/ob mice, which indicate of Purendan has the exact effect of intervention on the development of pre-diabetes to type 2 diabetes. |
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