|
| Research on the single nucleotide polymorphism of Aβ and CypD in brain tissue of senescence accelerated mice and the intervention effect of triple energizer techniques of needling |
| Hits 2039 Download times 1401 Received:October 26, 2016 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2017.03.13 |
| Key Words
Alzheimer's disease;single nucleotide polymorphism;Aβ;CypD |
| Author Name | Affiliation | E-mail | | HAN Xiu-di | Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FU Yu | The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | happyfu1970@163.com |
|
| Abstract
|
| [Objective] To study the differential expression of Aβ and CypD in hippocampal and cortical neurons of senescence accelerated mice SAMP8 and the same strain normal mice SAMR1 were caused by single nucleotide polymorphism (SNP) in their gene CDs regions, DNA sequence, and to study if the triple energizer techniques of needling has intervention effect on DNA sequence of Aβ and CypD CDs regions in hippocampal and cortical neurons of senescence accelerated mice SAMP8 and the same strain normal mice SAMR1.[Methods] Senescence accelerated mice SAMP8 was selected as the model, using PCR amplification and SNP generation sequencing method to compare DNA sequences of Aβ and CypD CDs primer design sites in their cortex and hippocampus and to observe intervention effect of triple energizer techniques of needling on DNA sequence of Aβ and CypD CDs regions.[Results] SNP were not found in DNA sequences of CDs primer design sites of SAMR1 and SAMP8 mice's cortex and hippocampus, and no changes were found in DNA sequences of CDs primer design sites of acupuncture group mice's cortex and hippocampus.[Conclusion] No relationship was found between of Aβ and cypd mRNA and protein differential expression and SNP in DNA sequence of their gene CDs regions and no intervention effect of triple energizer techniques of needling on DNA sequence of Aβ and cypd gene CDs regions. Furthermore, it is speculated that there was no relationship between the occurrence of AD and SNP in DNA sequence of Aβ and cypd CDs regions and that triple energizer techniques of needling is not by changing the DNA sequence of Aβ and cypd CDs regions to regulate mRNA and protein expression levels of Aβ and cypd. |
|
