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| Effects of Xiaokeling tablet on JNK signaling pathway with type 2 diabetic |
| Hits 1516 Download times 1339 Received:October 27, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.04.13 |
| Key Words
Xiaokeling tablets;diabetic rats;JNK signaling pathway;PDX-1 protein |
| Author Name | Affiliation | | WANG Shi-wei | The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | XU Ning | The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To study the effect of Xiaokeling tablets (XKL) on JNK signaling pathway in type 2 diabetic rats. [Method] Preparation of type 2 diabetic rat model by using high-sugar and high-fat food feeding combined with low-dose streptozotocin (STZ). Experimental animals were divided into normal control group, model group, XKL low, medium and high dose group (intragastric administration in low, medium and high dose group as 2, 4, 8 g/kg respectiely), positive control group (intragastric administration by 0.8 g/kg metformin hydrochloride). Fasting blood glucose (FBG), fasting insulin (FINS), malondialdehyde (MDA) level, superoxide dismutase (SOD) activity, phosphorylation of c-Jun N-terminal kinase (p-JNK), pancreas duodenal homeobox-1 (PDX-1) protein relative expression and JNK mRNA expressionrats were measured in each groups of rats. [Result] The level of FBG and MDA were effectively reduced and the level of FINS and SOD activity were improved in XKL each dose groups. The middle and high dose group had statistical significance compared with the model group (P<0.05). The expression of p-JNK protein and JNK mRNA was significantly decreased in middle and high dose compared with the model group (P<0.05). The expression of PDX-1 protein increased significantly in middle and high dose compared with the model group (P<0.05). [Conclusion] The hypoglycemic effect of XKL may be related to inhibiting the activation of JNK signaling pathway. |
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