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| Simultaneous determination of three flavonoids in hawthorn leaves extract by HPLC-MS/MS method and analysis of their pharmacokinetics in plasma of rats |
| Hits 1746 Download times 1578 Received:November 14, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.04.16 |
| Key Words
pharmacokinetics;HPLC-MS/MS;hawthorn leaves extract |
| Author Name | Affiliation | E-mail | | MA Hao-ling | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG De-qin | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | deqin123@163.com | | DING Li-qin | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHEN Xin-ze | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WU Xiao lei | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WU Ming-jing | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | QIU Feng | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To establish a HPLC-MS/MS method for the stimultancous pharmacokinetic study of vitexin-4“-O-glucoside(VOG), vitexin-2”-O-rhamnoside(VOR) and vitexin(VIT) in rat plasma after administration of hawthorn leaves extract orally. [Methods] Samples of plasma were obtained at different time after rats were administrated with three doses of hawthorn leaves extract (1, 2, 4 g/kg). After the deproteinization by methanol, extract of plasma samples were separated on a Agilent ZORBAX XDB-C18 column (3.5 μm,2.1×50 mm) with gradient elution using methanol and water (containing 0.1% formic acid) as mobile phase. Mass spectrometric detection was carried out by multiple reaction monitoring (MRM) using electrospray ionization in negative ion mode. [Results] A good linearity of VIT, VOG and VOR was shown in the ranges of 0.50~500.0 ng/mL (r=0.998), 1.0~1000.0 ng/mL(r=0.999) and 1.0~1000.0 ng/mL(r=0.998), respectively. The methods of precision, accuracy, recovery and stability are in line with the requirements of determination of biological samples. [Conclusion] The method is proved to be simple, rapid, and specific, and to be suitable for the simultaneous determination of VIT, VOG and VOR in plasma of rats and the pharmacokinetic study. |
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