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| Study of protective mechanism of calycosinon on myocardial ischemia-reperfusion injury in rats |
| Hits 1714 Download times 1294 Received:January 12, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.05.16 |
| Key Words
calycosin;ischemia-reperfusion;Nrf2/HO-1 signaling pathway |
| Author Name | Affiliation | E-mail | | LI Chun-jie | Tianjin Chest Hospital, Tianjin 300222, China | | | XING Xiao-xue | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHOU Zheng-can | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YU Jia-qi | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FAN Guan-wei | Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | fgw1005@hotmail.com |
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| Abstract
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| [Objective] To study the protective mechanism of calycosinon myocardial ischemia-reperfusion injury in rats.[Methods] Myocardial ischemia reperfusion injury model established using the Langendorff device. The parameters of cardiac function were monitored in real-time; the level of LDH and CK in coronary effluent fluid, MDA and SOD in heart tissue were detected. The pathological damage of myocardial tissue was observed by staining with TTC. At the same time, the effects of calycosin on the protein expression of Nrf2, HO-1 were investigated by Western blot.[Results] Calycosincan significantly increase coronary flowrate, left ventricular development pressure, maximal ascending/descending rate, and improved cardiac function in the model of ischemia-reperfusion injury, decreased the levels of CK and LDH in coronary effluent, while increased antioxidant enzymes activity such as MDA, SOD and SDH including downstream regulation of the antioxidant genes by activating the Nrf2 gene.[Conclusion] Calycosin played a role in anti-oxidativestress by activating the Nrf2/HO-1 signaling pathway, and then protected myocardial from ischemic-reperfused injury. |
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