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| Simultaneous determination of columbianetin-β-D-glucopyranoside and columbianetin in Angelicae pubescentis radix by HPLC method |
| Hits 1543 Download times 1338 Received:December 28, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.05.17 |
| Key Words
Angelicae pubescentis radix;columbianetin-β-D-glucopyranoside;columbianetin;HPLC |
| Author Name | Affiliation | E-mail | | ZHANG Lu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GE Yuan-yuan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Jin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yan-xu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tcmcyx@126.com |
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| Abstract
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| [Objective] To research the best extraction process about columbianetin-β-D-glucopyranoside and columbianetin in Angelicae pubescentis radix and develop a simple, accurate, repeatable method for simultaneous determination of columbianetin-β-D-glucopyranoside and columbianetin in Angelicae pubescentis radix by High Performance Liquid Chromatography(HPLC).[Methods] The sample was extracted by different extraction solvent, extraction time, ratio of liquid to material to analysis the effect about the content of columbianetin-β-D-glucopyranoside and columbianetin.[Results] The optimum level were methanol, 60 min, 1:50, respectively. Two calibration curves exhibited good linearity (r=1) within the linear range from 0.312 5 μg/mL to 20 μg/mL. The average percentages of recovery was 99.0% and 102%, and the RSD was 2.99% and 2.59%, respectively.[Conclusion] The developed simple HPLC method could successfully be utilized to determine two components in samples from different regions. It was concluded that this new method could determine columbianetin-β-D-glucopyranoside and columbianetin for quality control of Angelicae pubescentis radix. |
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