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| Simultaneous determination of three active components in decoction of rhizoma cimicifugae and radix puerariae by HPLC |
| Hits 1843 Download times 1228 Received:October 21, 2016 |
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| DOI
10.11656/j.issn.1672-1519.2017.05.18 |
| Key Words
decoction of rhizome cimicifugae and radix;HPLC;isoferulic acid;puerarin;paeoniflorin |
| Author Name | Affiliation | E-mail | | YAO Shao-zi | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Mimstry Co-established state Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Meng-chun | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Mimstry Co-established state Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LU Peng | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Mimstry Co-established state Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Jin-yan | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Mimstry Co-established state Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHEN Shi-bin | China Resources of Sanjiu Medical & Pharmaceutical Co. Ltd. Shenzhen 518110, China | | | LIU Jing-jing | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Mimstry Co-established state Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Zhi-dong | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Mimstry Co-established state Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | lonerliuzd@163.com |
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| Abstract
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| [Objective] To develop an analytical method for simultaneously determining the contents of paeoniflorin, puerarin and isoferulic acid in decoction of rhizoma cimicifugae and radix puerariae by HPLC.[Methods] The separation of three active components were performed on a Waters XBridge column (250 mm×4.6 mm, 5 μm) by using an gradient mobile phase composed of CH3OH-0.05 mol/L KH2PO4, at acolumn temperature of 30℃, the flow rate was 0.8 mL/min, the detection wavelength was 230 nm and 317 nm.[Results] The peaks of paeoniflorin, puerarin and isoferulic acidcompletely resolved very well. The linear ranges of paeoniflorin, puerarin and isoferulic acid were 0.382~6.118 μg, 1.602~25.626 μg and 0.081~1.303 μg, the average recoveries(n=6) were 101.5%, 102.9% and 98.1% respectively, and the RSD was less than 3.0%.[Conclusion] This method is simple, accurate and repeatable. It can be used to simultaneously determine the content of the above three active compounds, as well as to provide a scientific basis for the quality evaluation of the decoction. |
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