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| Experimental research on conjunctivitis treated with Qingre Mingmu decoction by ultrasonic atomization method |
| Hits 1423 Download times 1135 Received:March 20, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.06.15 |
| Key Words
Qingre Mingmu decoction;ultrasonic atomization;conjunctivitis |
| Author Name | Affiliation | E-mail | | WANG Qi-miao | China-Japan Friendship Hospital, Beijing 100029, China | | | JIN Ming | China-Japan Friendship Hospital, Beijing 100029, China | jinming57@163.com | | PAN Lin | China-Japan Friendship Hospital, Beijing 100029, China | | | XIANG Zhan-mei | China-Japan Friendship Hospital, Beijing 100029, China | | | QIN Ya-li | China-Japan Friendship Hospital, Beijing 100029, China | |
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| Abstract
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| [Objective] To take the study on efficacy and the mechanism of the conjunctivitis model with Qingre Mingmu decoction by ultrasonic atomizing method.[Methods] Put Qingre Mingmu decoction as test samples, and the left Ofloxacin Eye Drops as control. Establish rabbit conjunctivitis model with micro-injector trickle of cantharid in tincture. Then observe the eye irritation symptom, conjunctiva histopathology and the expression of IL-1beta, IL-6, TNF-alpha by immunohistochemical staining of every group. [Results] Each group determine the successful model and immediately corresponding treatment. Comparing with the model group, pathological histological observation of Qingre Mingmu concentration group and the control group had significant difference (P<0.05). IL-1beta control group and traditional Chinese medicine concentrations less expression in the epithelial cell layer, but both better than the model group.IL-6 and TNF-alpha were mainly expressed in the epithelial cell layer, Model group on the cortical expression of the most obvious difference. [Conclusion] There was obvious effect on Qingre Mingmu ultrasonic atomization method treating conjunctivitis. Immunohistochemical localization of rabbit conjunctivitis model of inflammatory factors of IL-1 β, IL-6 and TNF- α, and qualitative analysis, show that there is a close relationship between conjunctivitis and the factors. The severity of inflammatory factor expression can reflect the conjunctival disease. |
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