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| Anti isoprenaline-induced cardiac hypertrophy effect of Jatrorrhizine hydrochloride on H9c2 cells |
| Hits 1705 Download times 1361 Received:March 04, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.06.16 |
| Key Words
jatrorrhizine hydrochloride;cardiac hypertrophy;isoprenaline;Ca2+;ANP;BNP;β1 adrenal receptor |
| Author Name | Affiliation | E-mail | | NIU Zi-chang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GAO Yun-hang | Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Xing-ye | Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MAO Hao-ping | Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | haoping_mao@126.com |
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| Abstract
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| [Objective] To investigate the effect of jatrorrhizine hydrochloride on isoprenaline (ISO) induced cardiac hypertrophy in H9c2 cells. [Methods] H9c2 cells were cultured in vitro. Using the quantity of total proteins in cells to evaluate the level of cardiac hypertrophy; Real-time PCR was used to investigate the effect of jatrorrhizine hydrochloride on increase of ANP and BNP mRNA induced by ISO. Calcium-4 kit was used to detect the concentration of Ca2+ in cells. After transfect β1 adrenal receptor into cells, the binding abilities of jatrorrhizine hydrochloride and ISO to β1 adrenal receptor were examined. Western blot was used to detect the effect of jatrorrhizine hydrochloride on activation of ERK pathway. [Results] Jatrorrhizine hydrochloride significantly inhibited H9c2 cell hypertrophy induced by ISO. Jatrorrhizine hydrochloride significantly inhibited the increase of ANP and BNP mRNA. Jatrorrhizine hydrochloride showed milder bind ability to β1 adrenal receptor compared with ISO. Jatrorrhizine hydrochloride significantly inhibited ISO induced ERK pathway activation. [Conclusion] Jatrorrhizine hydrochloride can inhibit ISO induced cardiac hypertrophy by binding to β1 adrenal receptor. |
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