|
| Determination of total flavonoid glycosides in Shuxuening injection by HPLC |
| Hits 1411 Download times 1197 Received:February 05, 2017 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2017.06.17 |
| Key Words
Shuxuening injection;quercetin;kaempferol;rhamnetin;total flavonoid glycosides |
| Author Name | Affiliation | E-mail | | TIAN Ji | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Jin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Jun | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GUAN Xiu-wei | Hebei Shineway Pharmaceutical Co. Ltd., Langfang 065200, China | | | ZHANG Gang | State-Local Joint Engineering Laboratory of New Drugs Department Technology for Traditional Chinese Medicine Injection, Langfang 065200, China | | | LI Zhi-gang | Hebei Province Engineering Research Center for Traditional Chinese Medicine Injection, Langfang 065200, China | lizhigang@shineway.com | | CHANG Yan-xu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tcmcyx@126.com |
|
| Abstract
|
| [Objective] To establish a high performance liquid chromatography (HPLC) method to determine the content of total favonol glycosidesfor quality control of Shuxuening injection. [Methods] The HPLC analysis was performed on an Extend-C18 column (4.6×250 mm, 5 μm). Acetonitrile and water containing 0.1% formic acidwas selected asmobile phrasein a gradient elution at a flow rate of 1 mL/min. The injection volume was 15 μL.The column temperature was set up at 35℃.The detection wavelength was 360nm. [Results] There were good separation and the linear relationships of quercetin、kaempferol and rhamnetin. The RSD of precision, the repeatability and stability all were less than 5%. The sample recoveriesranged from 99.5% to 100% and their RSD values were less than 5%.The averagecontent of total flavonoid glycosides in 30 batches ofShuxuening injections was 1.06 mg/mL and there was no significantly different from different batches. [Conclusion] The method could be applied to determinethe total content of flavonoid glycosides of Shuxuening injection accurately and reliably, which provide a scientific basis for the quality evaluation of Shuxuening injection. |
|
|
|
|
|
|
|
