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| Effect of medicated serum prepared with Shugan Mingmu decoction on choroidal neovascularization by HIF1α-VEGF-Notch1 pathway |
| Hits 1398 Download times 1423 Received:February 26, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.07.12 |
| Key Words
medicated serum prepared with Shugan Mingmu decoction;age-related macular degeneration;choroidal neovascularization;choroidal endothelial cell;hypoxia-inducible factor 1 |
| Author Name | Affiliation | | JIAO Yi | Department of Ophthalmology, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | ZHANG Xue-zhu | Department of Ophthalmology, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China |
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| Abstract
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| [Objective] In this study, the potential effect of medicated serum prepared with Shugan Mingmu decoction on proliferation ability of rat choroidal endothelial cells (CECs) and HIF1α-VEGF-Notch1 dependent regulatory mechanisms were investigated. [Methods] CECs cells were cultured in normoxic and hypoxic conditions. The effects of medicated serum prepared with Shugan Mingmu decoction on proliferation and migration abilities of CECs were detected. The expression levels of HIF1α, VEGF and Notch1 mRNA and protein were determined by RT-PCR and western blot. [Results] Compared with the control group, the proliferation and migration abilities of CECs in the hypoxia group were significantly promoted (P<0.01), and significant increased HIF1α, VEGF and Notch1 mRNA and protein expression were also detected (P<0.01). After treated with medicated serum, the migration and migration abilities of CECs decreased obviously when compared with the hypoxia group (P<0.01). The mRNA and protein expression levels of HIF1α and VEGF were decreased (P<0.01 and P<0.05), whereas the expression of Notch1 was increased further (P<0.01). [Conclusion] Medicated serum prepared with Shugan Mingmu decoction could inhibit CECs proliferation and migration by up-regulating the expression of Notch1 and down-regulating the activity of HIF1α-VEGF signal pathway, and thus decreased the formation of choroidal neovascularization. |
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