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| Effect of total saponins from Rhizoma Dioscorea nipponica on biomarkers in urine of gouty arthritis rats |
| Hits 1653 Download times 1311 Received:February 20, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.07.16 |
| Key Words
total saponins from Dioscorea nipponica Makino;gouty arthritis;guanosine;creatinine;uric acid;HPLC |
| Author Name | Affiliation | E-mail | | LIN Fang-fang | Graduate School, Heilongjiang University of Chinese Medicine, Harbin 150040, China | | | ZHOU Qi | Research Institute of Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China | | | LIU Shu-min | Technology Innovation Team of High Education in Heilongjiang Province, Harbin 150040, China | keji-liu@163.com |
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| Abstract
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| [Objective] To explore the effect of total saponin of Rhizoma Dioscorea nipponica (RDN) on contents of guanosine, uric acid and creatinine in urine of gouty arthritis rats by high performance liquid chromatography (HPLC) with UV detection and UV spectrophotometric. [Methods] The 60 Wistar rats were randomly divided into six groups. They were normal group, model group, total saponins groups of high (160 mg/kg) ,middle (80 mg/kg) and low(40 mg/kg) doses and colchicine group. Total saponins of RDN were given for 7 successive days. An hour after total saponins of RDN were given at the third day, 0.2 mL, 25 mg/mL MSU suspension was injected into articular cavity through anadesma of kneecap in the knee-joint to induce model of gouty arthritis. HE and eosin dyeing were used to observe the histopathological change. A Diamonsil C18 column(5 μm, 250 mm×4.6 mm) was used for the analysis at 25℃, The separation was carried out with the mobile phase consisting of methanol-ammonium acetate(0.03 mol/L) at a flow rate of 1mL/min. The eluates were monitored by the programmed wavelength at 254 nm for guanosine; The contents of uric acid and creatinine in urine were detected by UV spectrophotometric. [Results] In the model group, the levels of guanosine and creatinine were significantly decreased and the content of uric acid was significantly increased. Compared with the model group, guanosine, uric acid and creatinine contents increased significantly in total saponins groups. [Conclusion] Guanosine, creatinine and uric acid were important biological markers in the occurrence and development of gouty arthritis. Total saponin of RDN have potential effects on the treatment of gouty arthritis by regulating the content change of these biomarkers. |
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