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| Study on bile excretion of pinoresinol diglucoside of rats |
| Hits 1351 Download times 1212 Received:May 20, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.07.17 |
| Key Words
pinoresinol diglucoside;LC-MS/MS;biliary excretion;metabolite |
| Author Name | Affiliation | E-mail | | HAN Ya-ya | Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Lei | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Wen-jie | Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HAO Jia | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Er-wei | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | liuwei628@hotmail.com |
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| Abstract
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| [Objective] To establish a method for simultaneous determination of pinoresinol diglucoside and its metabolites pinoresinol singleglucoside in rat bile by liquid chromatography-mass spectrometry (LC-MS/MS) and to study the excretion of two compound from rat bile. [Methods] Bile samples were collected according to the designed time periods after intragastric administration. The content of pinoresinol diglucoside, with the warfarin prime as internal standard, and it's metabolites in rat bile were then analyzed using the established LC-MS/MS method. [Results] Calibration curves were generated over a concentration range of 8~5 000 ng/mL for pinoresinol diglucoside and pinoresinol singleglucoside, respectively. Interday, intra-day and other methodology study RSDs were in accordance with the requirements of the biological samples quantitatively. Biliary accumulated excretion after administration were growing continually, to maintain a steady state after 6 hours; discharge rate in 4 hours to reach the top, afterdischarge rate falling rapidly, after 6 hours in a low condition,rats excretion rate has a rasing trend in the late experiment.[Conclusion] This method meets the requirements for the quantitative determination of biological samples and can be used forquantitative analysis of pinoresinol diglucoside and it's metabolites pinoresinol singleglucosidein rat bile. |
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