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| Establishment of HPLC maxplot chromatographic fingerprints of Rhizoma Coptidis |
| Hits 1932 Download times 1330 Received:February 21, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.08.20 |
| Key Words
Rhizoma Coptidis;PDA MaxPlot;accompany reference;fingerprinting;quality evaluation |
| Author Name | Affiliation | E-mail | | ZHANG Chun-yan | Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Department of Pharmacy, Tianjin Binhai New Area of Traditional Chinese Medicine Hospital, Tianjin 300451, China | | | CHENG Rui-zhen | Department of Pharmacy, Tianjin Binhai New Area of Traditional Chinese Medicine Hospital, Tianjin 300451, China | 337630245@qq.com | | LIU Hai | Department of Pharmacy, Tianjin Binhai New Area of Traditional Chinese Medicine Hospital, Tianjin 300451, China | |
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| Abstract
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| [Objective] To develop the chromatographic fingerprints of Rhizoma Coptidis based on Maxplot technique by high performance liquid chromatography with photodiode array detector (HPLC-PDA) and provide basis for the comprehensive and effective quality control of Rhizoma Coptidis.[Methods] The HPLC method was performed on an Agilent SB-C18(4.6 mm×150 mm, 3.5 μm)by a gradient elution using acetonitrile and 0.1% trifluoroacetic acid aqueous solution as the mobile phase;A MaxPlot chromatogram extracted from PDA data obtained from 210 to 400 nm. All peaks in the Maxplot chromatogram of Certified Reference Materials for Rhizoma Coptidis tested simultaneously were taken as the reference fingerprint index. The quality of Rhizoma Coptidis was evaluated by similarity analysis of chromatographic fingerprint.[Results] The results indicated that 11 common peaks were detected in the chromatographic fingerprint by using MaxPlot techinique, which significantly improved the performance of fingerprint compared with that at 345 nm.[Conclusion] By MaxPlot technique, the sensitivity and the number of common peaks of fingerprint were improved, which could be conduced to the quality control of Rhizoma Coptidis comprehensively. |
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