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| Study on Guanmaitong and its components in invigorating the kidney and activating blood circulation action with VDR and CYP27B1 |
| Hits 1485 Download times 1442 Received:April 23, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.09.17 |
| Key Words
Guanmaitong tablet;VDR;CYP27B1;invigorating the kidney and activating blood circulation action |
| Author Name | Affiliation | E-mail | | LV Nan | Tianjin Instuute of Meddical and Paramaceutical Sciences, Tianjin 300020, China | | | SHEN Yu-yan | Tianjin Tongrentang Group Limited Company, Tianjin 300020, China | | | LIU Hong-bing | Tianjin Instuute of Meddical and Paramaceutical Sciences, Tianjin 300020, China | | | WANG Jia | Tianjin Tongrentang Group Limited Company, Tianjin 300020, China | | | WANG Lei | Tianjin Instuute of Meddical and Paramaceutical Sciences, Tianjin 300020, China | | | ZHANG Yi | Tianjin Nankai Hospital, Tianjin 300010, China | | | DU Xue-mei | Tianjin Tongrentang Group Limited Company, Tianjin 300020, China | lvnantj@sina.cn |
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| Abstract
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| [Objective] To investigate the role of Guanmaitong tablets and its components in invigorating the kidney and activating blood circulation action with VDR and CPY27B1.[Methods] The model of myocardial infarction rats with kidney yang deficiency was established by adenine and ligation of the left anterior descends in branch.The rats were divided into sham operation group,myocardial infarction kidney yang deficiency model group,Guanmaitong tablets whole components group,invigorating the kidney components group, activating blood circulation components group and the vitamin D group.Precautionary administered orally prior to AMI patients 2 d,then administration 5 d,1 time of each day after model.Serum creatinine (Scr) and T3,T4 levels are detected.ELISA measurement of serum activation 1,25(OH)2D3 level.Immunohistochemical staining was observed in ventricular muscle tissue,kidney tissue vitamin D receptor (VDR),kidney tissue 1α-hydroxylase (CYP27B1) expression.Western blot assay ventricular muscle tissue,kidney tissue and kidney tissue CYP27B1 and VDR protein relative expression.[Results] Guanmaitong tablets whole components can reduce the Scr significantly (P<0.05),increase T3,T4 effect significantly (P<0.05) and increase serum 1,25(OH)2D3 level (P<0.05).The whole components group can increase the expression of CPY27B1 in renal tissue.The whole components and its part components can also increase VDR expression in cardiac and kidney and renal tissues.[Conclusion] Guanmaitong tablets may act on CPY27B1 and VDR as a core part of its mechanism for invigorating the kidney and activating blood circulation action to provide data supporting basic research. |
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