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| Study on establishing specific chromatogram of Sophora alopecuroides L. polysaccharides by pre-column derivation HPLC method |
| Hits 1508 Download times 1179 Received:March 25, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.09.18 |
| Key Words
Sophora alopecuroides L. polysaccharides;pre-column derivation;HPLC;specific chromatogram;monosaccharide |
| Author Name | Affiliation | E-mail | | ZHANG Yan | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | ZHAO Zhen-yu | Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | | | WANG Ping | Tasly Holding Group, Tianjin 300410, China | | | ZHANG Gui-xian | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | FU Yu | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | LIU Qing-Huan | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | TAO Zun-wei | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | taozunweitj@163.com |
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| Abstract
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| [Objective] To establish specific chromatogram of Sophora alopecuroides L. polysaccharides.[Methods] The monosaccharide composition of Sophora alopecuroides L. polysaccharides was determined by pre-column derivation HPLC method.[Results] There were 7 peaks from the fingerprint which were mannose, glucose, rhamnose, galactose, xylose arabinose and an unidentifiable monosaccharide respectively.[Conclusion] The pre-column derivation HPLC method establishing specific chromatogram is a simple, accurate and reproducible. And the study can provide evidence for quality evaluation of Sophora alopecuroides L. polysaccharides. |
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