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| Effect of Danbai plastics anti-oxidantion and SCF/C-kit protein expression in cholasma rat model |
| Hits 1877 Download times 1145 Received:April 10, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.10.16 |
| Key Words
Danbai plastics;chloasma;antioxidation;SCF/C-kit protein expression |
| Author Name | Affiliation | E-mail | | GUO Dai-jiong | Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518021, China | | | SUN Jia-yu | Shenzhen Futian District Traditional Chinese Medicine Hospital, Shenzhen 518033, China | | | CHEN Bao-qing | Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518021, China | | | YANG Yu-feng | Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518021, China | | | GUO Ying | Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518021, China | | | HUA Nan | Department of Pharmacy, The Third Hospital of Beijing Army Police, Beijing 100141, China | 928389439@qq.com |
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| Abstract
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| [Objective] To investigate the oxidation resistance and SCF/C-kit protein expression of the Danbai plastics in cholasma rat model induced by progesterone and ultraviolet radiation.[Methods] The rats were randomly divided into control group, model Group, hydroquinone group, Danbai plastics group.The cholasma model was establised with progesterone (7.5 mg/kg) by im injection orultraviolet radiation in female rat. The effects of each group on GSH-Px, SOD, tyrosinase (TYR), T-AOC, MDA, NO, in skin were detected and physical examination of rat skin. The HaCat cells and melanocytes inhibition was observed under the action of different concentrations of Danbai extract (0,5,10,20,40,80 mg/mL) by CCK-8. According to the suitable inhibition concentration,Hacat cells were divided into 1640 media group,30 mJ/cm2 UVB exposure group,20 mg/mL Danbai extract group,30 mJ/cm2 UVB exposure combined with 20mg/mL Danbai extract group. The melanocytes were divided into MelM-2 media group,30 mJ/cm2 UVB exposure group,40mg/mL Danbai extract group,30 mJ/cm2 UVB exposure combined with 40 mg/mL Danbai extract group. The SCF expressions of Hacat cells as well as the C-kit expressions of melanocyte were detected by Western bolt.[Results] The result showed that the groups with the Danbai plastics could signifcantly increase the activity of GSH-Px, SOD and T-AOC, decrease the contents of MDA and TYR in skin. CCK-8 results showed that HaCat cells and melanocytes proliferated in a concentration-dependent inhibition and HaCat cells inhibition reached a peak at the concentration of 20 mg/mL (P<0.01) and the melanocytes reached a peak at the concentration of 40 mg/mL (P<0.01). The results also showed that Danbai extract inhibited the SCF expression of Hacat and the C-kit expression of melanocyte and it reduced the promotion of SCF/C-kit expressions by UVB.[Conclusion] The Danbai plastics have anti-oxidantion and suppress SCF/C-kit protein expression. |
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