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| Effects of Guchang capsules treatment and long chain and non coding RNA research on diarrhea-predominant of IBS in rat model |
| Hits 1953 Download times 1428 Received:May 28, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.11.14 |
| Key Words
Guchang capsule;diarrhea-predominant irritable bowel syndrome;Tnf-α;IL-6;lncRNA |
| Author Name | Affiliation | E-mail | | WANG Hong | Tianjin Medical University General Hospital, Tianjin 300052, China | | | CHEN De-you | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI li | Tianjin Zhongxin Pharmaceutical Group Limited by Share Ltd., Darentang Pharmaceutical Factory, Tianjin 300142, China | | | XV Chen-xia | Tianjin Zhongxin Pharmaceutical Group Limited by Share Ltd., Darentang Pharmaceutical Factory, Tianjin 300142, China | | | CHEN Ming-xin | Tianjin Zhongxin Pharmaceutical Group Limited by Share Ltd., Darentang Pharmaceutical Factory, Tianjin 300142, China | | | LIU Bao-shan | Tianjin Medical University General Hospital, Tianjin 300052, China | j33387@qq.com |
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| Abstract
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| [Objective] To investigate the effect of Guchang treatment and long chain and non coding RNA research on diarrhea-predominant IBS (D-IBS) in rat model.[Methods] Establish the rat model of diarrhea type irritable bowel syndrome. The rats were randomly assigned to normal group, model group, low dose of Guchang group, middle dose of Guchang group, high dose of Guchang group and pinaverium bromide group. The rat models of normal group and model group, received normal saline, The low dose, middle dose and high dose of Guchang groups were given the solution of Guchang with same concentration and different doses, the last pinaverium bromide group treated with Pinaverium ammonium dose. Observed the pathological results,the changes of TNF-α and IL-6 and the lncRNA in colon tissuein each group. [Results] 1)There was no obvious abnormal pathology in colonic of rats in each group. 2)The levels of TNF-α and IL-6 in colon tissue were significantly decreased and tended to be normal in middle dose and high dose of Guchang groups; the difference is proved to have statistical significance (P<0.05) Compared with pinaverium bromide group. 3)Randomly chose 4 (2 up-regulated, 2 down-regulated) differential expression lncRNAs. The results showed that in samples of normal group and model group, the 2 up-regulated lncRNAs showed relatively high expression in the model group (P<0.05, n=30), model group > low dose of Guchang > middle dose group of Guchang > high dose group of Guchang > pinaverium bromide group, in addition that the high dose group had no significant difference with pinaverium bromide group. The 2 down-regulated lncRNAs showed relatively high expression in normal group (P<0.05), normal group > low dose of Guchang > middle dose group of Guchang > high dose group of Guchang > pinaverium bromide group. There was a significant difference between the other two groups, in addition that the high dose group had no significant difference with pinaverium bromide group.[Conclusion] The occurrence and development of D-IBS has a close relationship with Inflammatory factor TNF-α, IL-6 and lncRNA. Guchang can treat D-IBS by regulating TNF-α, IL-6 and the expression level of specific lncRNA. It lays a theoretical and experimental foundation for the following research, and provides a reliable new theory and new target of Chinese medicine to treat this disease. |
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