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| Simultaneous determination of six bioactive components for quality control of Ramulus Mori by HPLC |
| Hits 1991 Download times 1370 Received:June 20, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2017.11.16 |
| Key Words
Ramulus Mori;liquid chromatography;Isoquercitrin;kaempferol |
| Author Name | Affiliation | E-mail | | LI Jin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | DU Kun-ze | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LUO Rong | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIN Hua | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Lin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yan-xu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tcmcyx@126.com |
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| Abstract
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| [Objective] To establish a high pressure liquid chromatography (HPLC) method for simultaneous determination of Mulberroside A, cryptochlorogenic acid, chlorogenic acid, rutin, Isoquercitrin and kaempferol content for quality control of Ramulus Mori.[Methods] The separation was carried out on a Agilent C18 column (4.6 mm×250 mm, 5 μm) column with the mobile phase of water (containing 0.1% formic acid)-acetonitrile. The detection wavelength was 360 nm. The flow rate was set at 1 mL/min. The content of six ingredients of Ramulus Mori were calculated by external standard method.[Results] This method exhibited a good linear relationship. Relative standard deviation (RSD) of stability was 1.04%~3.07%. The RSD of the reproducibility was 2.22%~4.70%. The precision RSD was 1.34%~4.90%. The average recovery was 97.4%~104.0% (RSD=1.01%).[Conclusion] The simple, accurate, sensitive method established could be used to evaluate the quality of Ramulus Mori. |
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