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| Study on the mechanism of the inhibitory action of Huangqi injection on apoptosis of hippocampal neurons in diabetic encephalopathy rats |
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| DOI
10.11656/j.issn.1672-1519.2017.12.13 |
| Key Words
Huangqi injection;diabetic;cognition dyfuction;neuronal apoptosis |
| Author Name | Affiliation | E-mail | | LI Hu-hu | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WEI Bing | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZENG Wen-yun | Ganzhou People's Hospital, Ganzhou 341000, China | | | SUO Yan-rong | Ganzhou People's Hospital, Ganzhou 341000, China | | | GAO Qing | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Yi-jing | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | SUN Ying-xin | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Yun-sha | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FAN Ying-chang | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YANG Lin | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | yanglin7809@sina.com |
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| Abstract
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| [Objective] To explore the effects of Huangqi injection on learning and memory abilities in diabetic rats and its mechanism research.[Methods] Forty male SD rats, except the blank group(n=10),were intraperitoneal injected Streptozotocin (60 mg/kg) to establish type 1 diabetic rats, the modeled rats were equally and randomly divided into the model group and Huangqi injection group. All drugs were administrated for 10 weeks. The behavior test were performed on the Morris water maze method, and then the rats were executed for blood and brain sampling. Blood glucose, insulin and acetylcholinesterase level were detected. The expression of Bcl-2 and Bax in the hippocampus was examined by quantitative real-time PCR. The method of Western blotting was used to measure the expression of AchE. The expression of Caspase-3 in the hippocampus nerve cells was examined immunohistochemistry.[Results] Huangqi injection obviously improved the learning and memory abilities of diabetic encephalopathy rats, reduced blood glucose and AchE level and incresed the insulin level. The expression of AchE and Caspase-3 protein were obviously reduced in the hippocampus of rats. The anti-apoptosis gene Bcl-2 mRNA epression was increased while the apoptosis Bax mRNA expression was decreased in hippocampal area in Huangqi injection group.[Conclusion] Huangqi Injection obviously improved the learning and memory abilities of diabetic rats, its mechanism may be related to reduce AchE level, inhibit neuronal apoptosis. |
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