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| Experimental research on the influence of total glucosides of paeonia on the proliferation, apoptosis and cell cycle of fibroblast-like synovial cells |
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| DOI
10.11656/j.issn.1672-1519.2017.12.14 |
| Key Words
total glucosides of paeonia;osteoarthritis;synovial inflammation;abnormal growth |
| Author Name | Affiliation | E-mail | | WANG Huan | China-Japan Friendship Hospital, Beijing 100029, China | | | WANG Qing-fu | The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China | qingpu-wang@sohu.com | | TANG Xue-zhang | China-Japan Friendship Hospital, Beijing 100029, China | | | DING Hai-tao | China-Japan Friendship Hospital, Beijing 100029, China | | | ZHANG Mei-li | China-Japan Friendship Hospital, Beijing 100029, China | | | ZHANG Dong | Beijing University of Chinese Medicine, Beijing 100029, China | | | GUO Yu-ru | Beijing University of Chinese Medicine, Beijing 100029, China | | | LIU Si-ting | Beijing University of Chinese Medicine, Beijing 100029, China | | | FAN Xiao-yan | Beijing University of Chinese Medicine, Beijing 100029, China | | | XU Jing | Beijing University of Chinese Medicine, Beijing 100029, China | | | WANG Wei-li | Beijing University of Chinese Medicine, Beijing 100029, China | | | DING Hao-bin | Beijing University of Chinese Medicine, Beijing 100029, China | |
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| Abstract
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| [Objective] To discuss the regulatory mechanism of total glucosides of paeonia (TGP) on proliferation, apoptosis and cell cycle of fibroblast-like synovial cells (FLS) in the process of osteoarthritis (OA).[Methods] With FLS being the object of action, Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was used to detect the proliferation of FLS, and flow cytometry (FCM) was used to detect the apoptosis and cell cycle.[Results] The concentration dependence of FLS was existed in the concentration of TGP. In addition, the inhibitory effect of TGP on FLS mainly through inducing late apoptosis, as well as prolonging G2 phase (P<0.05) and blocking S phase (P<0.05) in the cell cycle of FLS.[Conclusion] TGP could inhibit the abnormal growth of FLS by regulating its proliferation, apoptosis and cell cycle. The findings above laid the groundwork for confirming the regulatory mechanism and curative effect of active components of Chinese medicine. |
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