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| Toxicity evaluation of Radix Kansui on zebrafish larvae |
| Hits 1933 Download times 1511 Received:August 26, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.01.14 |
| Key Words
Radix Kansui;zebrafish;toxicity evaluation |
| Author Name | Affiliation | E-mail | | MEI Xue | Beijing Key Lab for Quality Evaluation of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | LUO Jun | Beijing Key Lab for Quality Evaluation of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | XIA Qing | Beijing Key Lab for Quality Evaluation of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | FENG Yaru | Beijing Key Lab for Quality Evaluation of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | SUO Yaran | Beijing Key Lab for Quality Evaluation of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | LI Erwen | Beijing Key Lab for Quality Evaluation of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | WANG Zhaoyi | Beijing Key Lab for Quality Evaluation of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | MA Zhiqiang | Beijing Key Lab for Quality Evaluation of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | LIN Ruichao | Beijing Key Lab for Quality Evaluation of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China | linrch307@sina.com |
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| Abstract
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| [Objective] To evaluate the toxicity of Radix Kansui extracted with different solvent on zebrafish larvae.[Methods] The Radix Kansui was extracted with different solvent continuously by Soxhlet extraction method. The maximal non-lethal concentration and minimum total lethal concentration of each extract for 72 h were determined by pre-experiment. The median lethal concentration (LC50) was calculated by lethal curve which was established with 6~8 drug concentrations that are set in accordance with pre-experiment. 10% lethal concentration (LC10), the minimum lethal concentration (LC1), 1/3 LC1 and 1/10 LC1 of the most toxic extract were set up as concentrations of toxicity characteristic observation experiment. The control group and the solvent control group were set up at the same time. The toxicity characteristics of zebrafish larvae were observed after 72 h exposure with different concentrations. The toxicity reversibility was observed after the larvae living in fish water 48 h.[Results] The toxicity order of Radix Kansui extracts was petroleum ether extract > dichloromethane extract > ethyl acetate extract > ethanol extract. The toxicity of Radix Kansui is mainly characterized as liver toxicity and gastrointestinal irritation. Liver toxicity was showed as hepatomegaly and liver degeneration. Hepatomegaly is irreversible, but liver degeneration is reversible.[Conclusion] We simultaneously evaluated the toxic effects and reversibility of Radix Kansui on heart, liver, kidney, nervous system and gastrointestinal tract using one model with one administration for the first time. The results confirmed that the toxicity of Radix Kansui was mainly derived from petroleum ether extracts. Radix Kansui can cause substantial damage to the liver and gastrointestinal tract. This study provides a reference for the application of zebrafish in the evaluation of traditional Chinese medicine toxicity. |
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