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| Study on the cellular uptake and the up-take mechanism of cubic liquid crystalline nanoparticle on MDCK-MDR1 cells model |
| Hits 1689 Download times 1655 Received:July 26, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.01.15 |
| Key Words
MDCK-MDR1;LCNPs;uptake;mechanism;pathway inhibitor |
| Author Name | Affiliation | E-mail | | TAN Ning | School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | DU Shouying | School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | XUE Yutao | School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | TAN Li | School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China | | | LU Yang | School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China | landocean28@163.com | | LI Pengyue | School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China | pengyuelee@126.com |
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| Abstract
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| [Objective] To study cellular uptake and pathway of cubic liquid crystalline nanoparticles (LCNPs) on MDCK-MDR1 cells.[Methods] Make LCNPs with calein (Cal-LCNPs), the standard fluorescent substance. Fluorescence intensity in MDCK-MDR1 cells was measured by flow cytometry at different time points. Different inhibitors (Filipin, Phytoalexins D, 2-Deoxy-D-glucose, chlorpromazine) were incubated with Cal-LCNPs. Flow cytometry was used to determine the intracellular fluorescence intensity then determine the pathways.[Results] Cubic liquid crystal nanoparticles can not only increase the uptake of Cal in MDCK-MDR1 cells in 2h, but also alter the uptake behavior of cells. MDCK-MDR1 uptakes Cal was consistent with the zero-order equation, and Cal-LCNPs' was in accordance with the first-order equation. The intracellular fluorescence of cells incubated with chlorpromazine and 2-D-deoxyglucose was low conpared with control (P<0.05).[Conclusion] Cubic liquid crystal nanoparticles can increase the uptake of calcein by MDCK-MDR1, and the pathway is energy-dependent clathrin mediated endocytosis initiative. |
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