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| Research on improvement of lumbrokinas extracting process |
| Hits 2670 Download times 1926 Received:July 28, 2017 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2018.01.17 |
| Key Words
lumbrokinas;extraction;process improvement |
| Author Name | Affiliation | E-mail | | YU Qingbo | Hanlin Academy, Nanjing University of Chinese Medicine, Taizhou 225300, China | | | SU Pengliang | Hanlin Academy, Nanjing University of Chinese Medicine, Taizhou 225300, China | | | LIN Ying | Hanlin Academy, Nanjing University of Chinese Medicine, Taizhou 225300, China | | | LU Dongying | Hanlin Academy, Nanjing University of Chinese Medicine, Taizhou 225300, China | | | WANG Sainan | Hanlin Academy, Nanjing University of Chinese Medicine, Taizhou 225300, China | | | MA Yufan | Hanlin Academy, Nanjing University of Chinese Medicine, Taizhou 225300, China | | | XU Dan | Hanlin Academy, Nanjing University of Chinese Medicine, Taizhou 225300, China | xcjdan@163.com |
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| Abstract
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| [Objective] To improved extraction process of lumbrokinas.[Methods] Lumbrokinas was preparation from fresh earthworm by extraction, separation and purification. Sequential comparison of four solvent extraction methods, four precipitation separation methods and two chromatography purification methods, to obtaine a better process by investigating enzyme activity. Then the process was improved by fractional precipitation, removal of impurities and gel chromatography.[Results] Lumbrokinas was preparation by optimization of the extraction process, including buffer extraction, ethanol fractionation precipitation, DEAE-Sepharose FF and Sephadex G-75 chromatography. The enzyme activity was 28 000 μ/mg, which was higher than that of the old process.[Conclusion] The improved process has high enzyme activity and simple operation, and is suitable for industrial production. |
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