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| Protection of Cordycepin in pancreatic INS-1E cells against glucotoxicity |
| Hits 4431 Download times 1442 Received:December 04, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.02.18 |
| Key Words
glucotoxicity;Cordycepin;Akt;pancreas;INS-IE |
| Author Name | Affiliation | | ZHAO Haiyan | Shenzhen Nanshan District People's Hospital, Shenzhen 518052, China | | CHEN Libo | Shenzhen Nanshan District People's Hospital, Shenzhen 518052, China | | LI Liping | Shenzhen Nanshan District People's Hospital, Shenzhen 518052, China | | HUANG Xing | Shenzhen Nanshan District People's Hospital, Shenzhen 518052, China | | LI Jing | Shenzhen Nanshan District People's Hospital, Shenzhen 518052, China | | HU Yuanyuan | Shenzhen Nanshan District People's Hospital, Shenzhen 518052, China | | LYU Shuxin | Shenzhen Nanshan District People's Hospital, Shenzhen 518052, China |
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| Abstract
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| [Objective] To study the role of Cordycepin in its protection of pancreatic INS-1E cells against glucotoxicity.[Methods] To establish glucotoxicity INS-1Ecell model, the INS-1E cells viability was observed under the action of different concentrations of Cordycepin (6.125, 12.5, 25 μmol/L) by CCK-8, insulin secretion and synthesis was measured by ELISA Kit, phosphorylation of Akt was detected by Western-blot.[Results] Cordycepin improved viability in glucotoxicity pancreatic INS-1E cells, promoted insulin secretion and synthesis, increased phosphorylation of protein kinase Akt.[Conclusion] Cordycepin have protective effect in glucotoxicity pancreatic INS-1E cells. |
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