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| Rapid screening of estrogen-like active compounds from Rubus chingii Hu by Thermal Shift high throughput screening method |
| Hits 1727 Download times 2239 Received:November 08, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.03.18 |
| Key Words
hERα-LBD;hERβ-LBD;Rubus chingii Hu;ligand compound;estrogen-like activity |
| Author Name | Affiliation | E-mail | | ZHANG Zhongqun | Tianjin State Key Laboratory of Modern Chinese Medicine, Academy of Traditional Chinese Medical, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Jing | Tianjin State Key Laboratory of Modern Chinese Medicine, Academy of Traditional Chinese Medical, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Yongbin | Tianjin Acelbio Biological Technology Co., Ltd., Tianjin 300457, China | | | WANG Yu | Tianjin State Key Laboratory of Modern Chinese Medicine, Academy of Traditional Chinese Medical, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHAI Xin | Tianjin State Key Laboratory of Modern Chinese Medicine, Academy of Traditional Chinese Medical, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | chaixinphd@aliyun.com |
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| Abstract
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| [Objective] To screen estrogen-like active compounds from Rubus chingii Hu.[Methods] The subtypes of human estrogen receptor ligand binding domain (hERα-LBD and hERβ-LBD) were chosen as targets and E2 was set as positive control to screen compounds with estrogen-like activity from Rubus chingii Hu by using Thermal Shift high throughput method based on the ligand-receptor theory.[Results] Kaempferol can significantly increase the thermal stability of hERα-LBD and hERβ-LBD.[Conclusion] Kaempferol have an estrogen-like activity, which can bind to hERα-LBD and hERβ-LBD. |
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