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| Effect of Shenshuai No.1 on sensitive proteins KIM-1 and NGAL in kidney ischemia-reperfusion rats |
| Hits 1503 Download times 1195 Received:December 03, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.04.14 |
| Key Words
kidney ischemia-reperfusion;Shenshuai No.1;KIM-1;NGAL |
| Author Name | Affiliation | | KANG Li | Tianjin Institute of Medical and Pharmaceutical Sciences & Cardiovascular Drugs Research and Development Center, Tianjin 300020, China | | CHENG Guan | Tianjin Institute of Medical and Pharmaceutical Sciences & Cardiovascular Drugs Research and Development Center, Tianjin 300020, China | | WANG Zi | Tianjin Institute of Medical and Pharmaceutical Sciences & Cardiovascular Drugs Research and Development Center, Tianjin 300020, China | | JIA Shujie | Tianjin Institute of Medical and Pharmaceutical Sciences & Cardiovascular Drugs Research and Development Center, Tianjin 300020, China | | WANG Lu | Tianjin Haihe Hospital, Tianjin, 300350
Medical University of Tianjin, Tianjin 300070, China | | FAN Weiwei | Tianjin Academy of Traditional Chinese Medicine Afficiated Hospital, Tianjin 300120, China |
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| Abstract
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| [Objective] To investigate the effect of Shenshuai No.1 on the sensitive proteins KIM-1 and NGAL in kidney ischemia-reperfusion rats. [Methods] SD rats were randomly divided into 5 groups:sham operation group, model group, Niaoduqing granule positive group, high dose group and low dose group of Shenshuai No.1. In addition to the sham operation group, all the other groups underwent bilateral renal ligation and reperfusion, which resulted in acute ischemia-reperfusion injury. Continuous medication was used for 1 day, 3 days, 7 days after the operation. Serum urea nitrogen (BUN) and creatinine (SCr) were detected, the renal pathological morphology was observed, KIM-1 and NGAL of kidney were detected by Western Blot. [Results] Serum urea nitrogen (BUN) and creatinine (SCr) levels and the level of NGAL and KIM-1 in renal tissue of model group rats increased significantly (P<0.001). Compared with the model group, the level of serum BUN and SCr of Shenshuai No.1 high dose group and low dose group were significantly reduced (P<0.001; P<0.001, P<0.01), the protein expression of NGAL decreased significantly (P<0.01). HE staining showed that Shenshuai No.1 can reduce the occurrence and development of renal tubular epithelial cells, tubular cell necrosis, bleeding and inflammatory reaction, the effect of Shenshuai No.1 high dose was more obvious. [Conclusion] Shenshuai No.1 can protect the kidney by reducing the expression of KIM-1 and NGAL. |
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