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| Study on relation of seven flavonoids from mulberry leafs and their inhibition of α-glucosidase activities |
| Hits 1772 Download times 1388 Received:November 25, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.04.17 |
| Key Words
mulberry leafs;flavonoids ingredients;α-glucosidase inhibitory activity |
| Author Name | Affiliation | E-mail | | ZHU Ruichao | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LU Bin | College of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LUO rong | College of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Jin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Jun | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Tao | College of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Zhuan'ai | College of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yanxu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tcmcyx@126.com |
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| Abstract
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| [Objective] HPLC method was established for simultaneous determination of seven flavonoids ingredients and exploring the relationship between the total content of seven flavonoids and α-glucosidase inhibitory activity. [Methods] Orthogonal test was used to optimize extracting method of mulberry leaf. The HPLC method was established and validated to simultaneously determine seven flavonoids ingredients (mulberroside A, cryptochlorogenic acid, rutin, isoquercitrin, astragaline, quercitrin, kaempferol) in mulberry leaf. [Results] The optimal extracting conditions were that 0.5 g of mulberry leaf powder was extracted with 10 mL of 80% methanol (methanol-water) in ultrasonic bath for 30 min. The content of Rutin, Quercetin, Kaempferol, Isoquercitrin, Astragalin, Cryptochlorogenin acid, Mulberroside A are simultaneously determined by HPLC. [Conclusion] The established method is stable, repeatable and has good recovery. The seven flavonoids ingredients can be a reference for the strenth of α-glucosidase inhibitory activity. The results can provide a reference for quality control, clinical application, exploitation of mulberry leaf whose quality is better after Frost Desent |
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