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| Efficacy and mechanism of Jianpi Lishi decoction in the treatment of patients with mild to moderate ulcerative colitis by regulating the function of Treg cells |
| Hits 1490 Download times 1377 Received:December 10, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.05.07 |
| Key Words
ulcerative colitis;regulatory T cells;perforin;granzyme B |
| Author Name | Affiliation | | HAO Lili | Department of Spleen and Stomach Diseases, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | LIU Xiaoxi | Department of Spleen and Stomach Diseases, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] In this study, the clinical efficacy of Jianpi Lishi decoction on mild to moderate ulcerative colitis (UC) and mechanisms associated with the function of Tregs were investigated.[Methods] Altogether 59 patients with mild to moderate UC were enrolled and randomly allocated to the control and Jianpi Lishi groups. The control group received salbutamol enteric-coated tablets, and the Jianpi Lishi group was given Jianpi Lishi decoction orally. The treatment process would be lasted for 3 months. The main symptom scores, curative effect index and the quality of life of patients were observed before and after treatment. The concentrations of IL-1β and TGF-β1 in peripheral blood were measured by enzyme-linked immunosorbent assay (ELISA) and the mRNA levels of Foxp3, perforin and granzyme B were determined by RT-qPCR.[Results] After treatment, the above symptoms were improved significantly, and the effect of "Jianpi Lishi" group was much better than that of the control group (P<0.05). After 3 months of treatment, the quality of life in gastrointestinal symptoms, systemic symptoms, affective ability and social ability of the two groups were significantly higher than those before treatment (P<0.05), and the quality of life was significantly better in the Jianpi Lishi group when compared with the control group (P<0.05). Compared with normal subjects, the levels of IL-1β in peripheral blood were higher in the two groups before treatment, while the level of TGF-β was lower; the mRNA level of Foxp3 was lower, and the mRNA levels of perforin and granzyme B were higher (P<0.05 or P<0.01). After treatment, the levels of above-mentioned cytokines and genes were changed to the opposite direction with significant differences (P<0.05 or P<0.01), and the improvement effect of Jianpi Lishi group was more obvious than the control group (P<0.05).[Conclusion] Jianpi Lishi decoction can enhance the immunosuppressive function by regulating the number and function of Tregs, and inhibit the killing effect of perforin/granzyme B on target cells, so as to reduce the inflammatory reaction of intestinal tract. |
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