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| Mechanism research on the treatment of lymphoma with Xiaoluo pill based on PD-1/PD-L1 signaling pathway |
| Hits 1844 Download times 1668 Received:December 16, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.05.13 |
| Key Words
Xiaoluo pill;lymphoma;PD-1/PD-L1 signal pathway |
| Author Name | Affiliation | E-mail | | LIU Qunying | Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHANG Bo | Tianjin International Joint Academy of Biomedicine, Tianjin 300457, China | | | LI Haiyan | Dongzhimen Hospital of Beijing University of Traditional Chinese Medicine, Beijin 100700, China | | | DONG Lei | Dongfang Hospital of Beijing University of Traditional Chinese Medicine, Beijin 100078, China | | | SUN Chaoyi | Dongzhimen Hospital of Beijing University of Traditional Chinese Medicine, Beijin 100700, China | | | YANG Mingzhu | Dongzhimen Hospital of Beijing University of Traditional Chinese Medicine, Beijin 100700, China | | | SUN Song | Dongfang Hospital of Beijing University of Traditional Chinese Medicine, Beijin 100078, China | | | NI Lei | Beijing University of Chinese Medicine, Beijing 100029, China | nilei0108@163.com |
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| Abstract
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| [Objective] To investigate the immunoregulatory mechanism based on PD-1/PD-L1 signaling pathway in the treatment of Lymphoma with Xiaoluo pill.[Methods] Peripheral blood mononuclear cells (PBMC) were isolated from the peripheral blood of healthy people and lymphoma patients. After the cells were cultured in vitro, the activity of different subcellular cells of T cells was observed by whether to block the PD-1/PD-L1 signal pathway and drug intervention, which was detected by flow cytometry, CFSE and other technologies based on PD-1/PD-L1 signal target pathway.[Results] The expressions of PD-1 (+) and CD69 were decreased in CD4+ T cells and increased in CD8+ T cells in patients with T lymphoma. The inhibitory effect on CD69 in CD4+ cells in lymphoma T cells in combination therapy of PD-L1 blockade and Xiaoluo pill was significantly higher than that in PD-L1 blockade group and Xiaoluo pill group alone. Compared with the PD-L1 blockade group, the activity of CD8+ T cells increased significantly in Xiaoluo pill group. And compared with Xiaoluo pill group, the activity of CD8+ T cells in combination therapy of PD-L1 blockade and Xiaoluo pill was not significantly increased.[Conclusion] Xiaoluo pill could inhibit the high expression of PD-1 (+) on a large number of aberrant proliferative CD4+ T cells; both Xiaoluo pill and PD-L1 blockade have a synergistic effect on PD-1 (+) and CD69 expression in CD4+ T cells; Xiaoluo pill has a potent effect on CD8+ T cell activity and could promote PD-1 (+) expression. |
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