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| Inflammatory and neuropathic pain in mice reversed by Xihong Tongluo oral liquid |
| Hits 1444 Download times 1363 Received:December 26, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.05.14 |
| Key Words
Xihong Tongluo oral liquid;inflammatory swelling;inflammatory pain;stroke;neuropathic pain |
| Author Name | Affiliation | E-mail | | ZHANG Jian | Department of Pharmacology, School of Pharmaceutical Sciences, South-central University For Nationlities, Wuhan 430074, China | | | HUANG Fengzhen | Department of Pharmacology, School of Pharmaceutical Sciences, South-central University For Nationlities, Wuhan 430074, China | | | ZHANG Wei | Department of Pharmacology, School of Pharmaceutical Sciences, South-central University For Nationlities, Wuhan 430074, China | | | LI Xiaojun | Department of Pharmacology, School of Pharmaceutical Sciences, South-central University For Nationlities, Wuhan 430074, China | | | LI Yusang | Department of Pharmacology, School of Pharmaceutical Sciences, South-central University For Nationlities, Wuhan 430074, China | | | TANG Xiaoyi | Key Laboratory of Chinese Internal Medicine of MOE and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | | HU Yeyin | Key Laboratory of Chinese Internal Medicine of MOE and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | | SHANG Hongcai | Key Laboratory of Chinese Internal Medicine of MOE and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | | TANG Hebin | Department of Pharmacology, School of Pharmaceutical Sciences, South-central University For Nationlities, Wuhan 430074, China | | | TIAN Guihua | Key Laboratory of Chinese Internal Medicine of MOE and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | rosetgh@163.com |
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| Abstract
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| [Objective] To investigate the effect of anti-inflammatory and neurophatic pain of Xihong Tongluo oral liquid.[Methods] A dimethyl benzene-induced mouse auris swelling model was established to observe anti-inflammatory effects. The inflammatory pain was induced by a subcutaneous injection of 50 μL CFA into the bottom of left hind paws of mice. The response to writhing and stretching reaction induced by acetic acid (HAc, 0.6%, 50 μL) injection into a peritoneal cavity of mice was evaluated. A central poststroke pain (CPSP) model was used with a single collagenase (type IV; 0.25 μL, 0.025 U) injection into the left ventral posterolateral nucleus of the thalamus. Xihong Tongluo oral liquid was administrated to mice via intragastric administration and saline was administrated as a control. Then at corresponding time points (1/48 d, 1 d, 3 d, 5 d), inflammatory swelling, pain related-behaviors and histopathology were conducted and evaluated blindly.[Results] The experimental results of ear swelling showed that Xihong Tongluo oral liquid (low-dosage:20 L/g; high-dosage:100 L/g) exerted positive regulation in a time-dependent manner, in a comparison with model group. On the fifth day, ear edema of mice in the high-dose group was significantly alleviated compared with other groups. The edema of hind paw in CFA-inflamed mice was obvious on the first day. While significant reductions were seen in the group intragastric administrate with Xihong Tongluo oral liquid, while the high-dosage group showed the best anti-inflammatory effect; HE staining revealed that gavage treatment of Xihong Tongluo oral liquid can significantly alleviated the CFA-induced inflammatory infiltration. With gavage treatment, the number of writhing in HAc groups was significantly reduced, and high-dosage group showed significant analgesic effect on the 5th day. Nissl staining showed that CPSP weakened Nissl body staining and increased the amount of hypertrophic glial cells.[Conclusion] Those symptoms were improved after gavage treatment of Xihong Tongluo oral liquid. Xihong Tongluo oral liquid can relieve inflammatory swelling and pain response. While the injured neuron and hypertrophic glial cells induced by CPSP were improved, Xihong Tongluo oral liquid exerted greater anti-inflammatory and analgesic effects on central neuropathic pain. |
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