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| Experiment research of nourishing kidney and activating blood recipe to protect podocytes in focal segmental glomerulosclerosis mice |
| Hits 1608 Download times 1748 Received:February 18, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.05.15 |
| Key Words
Nourishing kidney and activating blood recipe;glomerulosclerosis;podocyte;wt1;nephrin;desmin |
| Author Name | Affiliation | E-mail | | ZUO Chunxia | Department of Nephrology, Tianjin Public Security Hospital, Tianjin 300042, China | | | ZHANG Mianzhi | Department of Nephrology, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | zhangmianzhi@vip.sina.com | | JIA Shengqin | Department of Nephrology, Tianjin Public Security Hospital, Tianjin 300042, China | |
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| Abstract
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| [Objective] To observe the effect of nourishing kidney and activating blood recipe on focal segmental glomerulosclerosis (FSGS) mice cytoskeleton protein desmin, slit diaphragm protein nephrin and renal tumor wt1, come to discuss the mechanism of the protective effect of podocyte.[Methods] The preparation of the FSGS nephropathy model was completed by the adriamycin induction model, and the treatment (set to the treatment group) was conducted after a week, and the duration of the treatment was six weeks. Then the blank control group and the model group were set up. In view of the three groups of kidney tissues and urine, blood, are collected, and detect the body quality, serum albumin (propagated), 24 hour(UAER), and urinary albumin/uric creatinine ratio (UACR), etc. Through the observation and analysis of the morphological changes of the renal tissue by the lens, the nephrin fluorescence intensity of the mice were determined by immunofluorescence. RT-PCR was used to detect desmin, wt1 mRNA expression levels in three groups of renal tissues.[Results] The UAER and UACR in the model group were significantly higher than that in the control group (P<0.01), and the quality of the model group and ALB were significantly lower than the control group and the treatment group (P<0.01). In the control group, nephrin immunofluorescence expression was characterized by continuous features, while the model group was characterized by a small number of points or flaky features. The expression of nephrin was significantly reduced in model group than of level of control group and treatment group. The expression levels of wt1 mRNA in the model group were significantly lower than those in the treatment group and the control group (P<0.01), and the expression levels of desmin mRNA were significantly higher than that in the control group and the treatment group (P<0.01).[Conclusion] The study concluded that the use of nourshing kidney and activating blood recipe group could reduce the renal tissue proteinuria in mice with FSGS, and may be associated with wt1, nephrin expression levels, and desmin expression levels. |
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