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| Andrographolide inhibits lipid droplet accumulation by blocking PPARγ-C/EBPα expression |
| Hits 2223 Download times 1330 Received:January 03, 2018 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2018.06.15 |
| Key Words
andrographolide;3T3-L1 adipocyte;PPARγ;C/EBPα;lipid metabolism |
| Author Name | Affiliation | E-mail | | LI Xi | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China | | | PENG Sha | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China | | | HOU Ning | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China | | | LIU Min | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China | | | LI Jing | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China | | | ZHANG Yuxin | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China | | | ZHANG Qiao | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China | | | WANG Shifeng | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China | | | ZHANG Yanling | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China | collean_zhang@163.com | | QIAO Yanjiang | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijin 100102, China | yjqiao@bucm.edu.cn |
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| Abstract
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| [Objective] To investigate the inhibitory effect of andrographolide on adipocyte differentiationand lipid droplet accumulation.[Methods] Different concentrations of andrographolide were added to 3T3-L1 adipocytes. The effect of andrographolide on the formation of fat droplets was performed by highcontent imaging. The molecular mechanism of andrographolide was illustrated by detecting the expression of PPARγ and C/EBPα.[Results] Andrographolide reduced lipid accumulation in dose-dependent manner. The expression of PPARγ and C/EBPα were significantly reduced at 30 μmol/L (P<0.01) without obvious cytotoxicity (P >0.05).[Conclusion] Andrographolide inhibited adipocyte differentiation through blocking PPARγ-C/EBPα expression and reduced excessive lipid droplets accumulation, which holds potential for alleviating obesity. |
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