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| Study on the interaction between BSA and psoralenoside as well as isopsoralenoside |
| Hits 1614 Download times 1248 Received:December 06, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.06.16 |
| Key Words
psoralenoside;isopsoralenoside;UV absorption spectroscopy study;fluorescence spectroscopy study |
| Author Name | Affiliation | E-mail | | GUO Jiading | Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HAO Jia | Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Mengrong | Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Yucheng | Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Erwei | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin 300193, China | liuwei628@hotmail.com |
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| Abstract
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| [Objective] To study of on the interaction between BSA and psoralenoside (PO) as well as isopsoralenoside (IPO).[Methods] Using the methods of UV absorption spectroscopy, fluorescence quenching spectroscopy, synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy quenching mechanism, binding constants, binding sites, conformational changes of PO as well as IPO to the BSA were analyzed.[Results] PO and IPO bound weakly to BSA with a static quenching process, which cause light changes in the polarity environment around the chromophore of tryptophan. The binding sites of the two drugs on BSA were Site Ⅱ.[Conclusion] The mechanism of interaction between PO and IPO with BSA was revealed through the spectroscopy study. |
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