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| Mechanism of naringin on the apoptosis of nucleus pulposus cells through Fas membrane receptor pathway |
| Hits 1394 Download times 1195 Received:January 11, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.06.17 |
| Key Words
naringin;human nucleus pulpous;apoptosis;intervertebral disc;degeneration |
| Author Name | Affiliation | E-mail | | YUAN Zhenzhen | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Hospital, Tianjin 300211, China | | | YANG Zhao | Tianjin Hospital, Tianjin 300211, China | | | JIN Hongbin | Tianjin Hospital, Tianjin 300211, China | annyallan@126.com | | XU Haiwei | Tianjin Hospital, Tianjin 300211, China | |
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| Abstract
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| [Objective] To study the effects of naringin on the apoptosis of nucleus pulposus cells of human degenerative intervertebral disc.[Methods] Nucleus pulposus cells were cultured in vitro and were identified via the safranin O and toluidine blue. Next, P3 cells were cultured with naringin 0 g/mL (control group), naringin group (20 g/mL, 40 g/mL, 80 g/mL). After 48 hours, the best concentration of naringin was selected by MTT for inhibition apoptosis of human degenerated nucleus pulposus cells and the rate of apoptosis in each group was measured by flow cytometry.[Results] The results of cells staining were positive, and it was proved that the cells cultured in the experiment were nucleus pulposus cells. MTT and flow cytometry were used to determine the optimum concentration of naringin (20 g/mL) to inhibit the apoptosis of nucleus pulposus cells(P<0.05). Naringin(20 g/mL) could inhibit the expression of Fas, FasL, Caspase-8 mRNA by qRT-PCR (P<0.05). Western-blot detection showed that 20 g/mL naringin could inhibit the expression of Fas, FasL, Caspase-8 protein.[Conclusion] Naringin may inhibit the apoptosis of nucleus pulposus cells by regulating the Fas/FasL death receptor apoptosis pathway. |
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