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| Effects of Rongchang capsule on autophagy-related proteins in epilepsy model induced by magnesium-free-treatment based on enriching kidney essence method |
| Hits 1846 Download times 1285 Received:February 20, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.07.15 |
| Key Words
epilepsy;enriching kidney essence method;magnesium-free;Beclin-1;Bcl-2 |
| Author Name | Affiliation | E-mail | | RONG Ping | Department of Pediatrics, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | PENG Bo | Tianjin University, Tianjin 300072, China | | | ZHANG Xilian | Department of Pediatrics, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHAO Wei | Department of Pediatrics, Xinjiang Yili State Hospital of Traditional Chinese Medicine, Yili 835000, China | | | ZHANG Xuerong | Department of Pediatrics, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061, China | | | WANG Wei | Department of Pediatrics, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Xuezhu | Department of Pediatrics, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Rong | Department of Pediatrics, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | mr1974@163.com |
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| Abstract
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| [Objective] To explore the autophagy regulation mechanism of antiepileptic and neuroprotective effects of Rongchang capsule to provide experimental evidence for clinical application.[Methods] Primary cultured neurons from hippocampus of neonate rats with recurrent epileptiform discharges induced by magnesium-free-treatment were divided into model group, 3-Methyladenine (3-MA) group, 5% concentration Rongchang capsule group, 10% concentration of Rongchang capsule group, 20% concentration of Rongchang capsule group, in addition, a normal group was set up. After giving corresponding treatment 6, 12, 24, 72 h, Western blot method was used to detect changes of the autophagy associated proteins (Beclin-1, Bcl-2, LC3B) expression.[Results] 1) Beclin-1 protein:6, 12, 24, 72 h Beclin-1 protein expression had no difference between model group and normal treatment group(P>0.05),6 h 20% concentration of the Rongchang capsule group and 72 h 5% concentration of the Rongchang capsule group enhanced significantly compared with model group(P<0.05). 2) Bcl-2 protein:6 h the model group Bcl-2 protein expression decreased significantly compared with the nomal group(P<0.05). 12, 24 h 20% concentration of Rongchang capsule group, and 72 h 5%, 10% and 20% concentration of Rongchang capsule group Bcl-2 protein expression enhanced significantly compared with model group (P<0.05 or P<0.01),20% concentration of the Rongchang capsule group Bcl-2 protein expression enhanced compared with 5% and 10% concentration of the Rongchang capsule group (P<0.05).[Conclusion] Rongchang capsule based on enriching kidney essence method may affect Beclin-1 protein expression and enhance Bc-l2 protein expression to play a role of antiepileptic and neuroprotective effects. |
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