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| Effects of Panax notoginsenosides on pharmacokinetics of Periplocin |
| Hits 1466 Download times 1407 Received:March 05, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.07.17 |
| Key Words
Periplocin;Panax notoginsenosides;LC-MS/MS;pharmacokinetics |
| Author Name | Affiliation | E-mail | | LIU Huaming | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Li | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Wenjuan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIA Qi | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | OUYANG Huizi | The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Jun | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | hejun673@163.com |
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| Abstract
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| [Objective] To establish a LC-MS/MS method for determination of Periplocin and study the effect of Panax notoginsenosides on pharmacokinetics of Periplocin in rats.[Methods] Eighteen rats were divided into Periplocin alone group; Periplocin combined with low-dose and high-dose Panax notoginsenosides groups. All rats were orally administered for seven consecutive days, blood samples were obtained from orbitalis at different time before and after the last time administration. LC-MS/MS method was developed and validated for the determination of Periplocin in rat plasma using psoralen as the internal standard, and main pharmacokinetic parameters of analyte were processed by DAS1.0 software.[Results] Calibration curves of Periplocin were linear over 1~500 ng/mL, the method validation was in line with requirements. Results of pharmacokinetics showed that there were significant differences between the pharmacokinetic parameters of Periplocin in rats with or without Panax notoginsenosides.[Conclusion] The LC-MS/MS method was rapid, sensitive and accurate, and it was successfully applied to evaluate the pharmacokinetics of Periplocin in rats. And the Panax notoginsenosides had effect on pharmacokinetic of Periplocin. |
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