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| Integral analysis of Dendrobium nobile and its extracts by infrared spectroscopy |
| Hits 1672 Download times 1144 Received:March 25, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.07.18 |
| Key Words
fourier transforminfrared spectroscopy;second derivative infrared spectroscopy;Dendrobium nobile Lindl.;overall structure analysis;extract |
| Author Name | Affiliation | E-mail | | HE Yuxin | School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | ZENG Yuxin | School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | ZHU Tiantian | School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | ZHU Nannan | School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | SUN Zhirong | School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | zrsun67@126.com |
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| Abstract
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| [Objective] Infrared spectroscopy was used to analyze the infrared spectra of the original medicinal materials of Dendrobium nobile and the chemical constituents contained in the two extracts.[Methods] Fourier transform infrared spectroscopy (FTIR) was used to analyze the structure of the Dendrobium nobile and the extracts of Dendrobium nobile.[Results] In the one-dimensional spectrum of Dendrobium nobile, the characteristic peaks of 2 923 cm-1, 2 855 cm-1, 1 738 cm-1, 1 155 cm-1, 1 081 cm-1, 1 019 cm-1 and so on can be seen. It is presumed that Dendrobium nobile contains lipids, aromatic and starches. The results of the second derivative of the original medicinal materials further confirmed the above results, while the strong peaks near 1 318 cm-1 and the characteristic peaks near 783 cm-1 appeared, and compared with the standard, it was determined to contain calcium oxalate. The peak of lipids, Aromatic and starches all appeared in the infrared spectra of extracts, but the number and the strength of peak were different. The strong characteristic peaks of starch were found in the one-dimensional and second-order derivative spectra of Dendrobium nobile, indicating that the compositions of water extract were mainly water-soluble polysaccharides, and the polysaccharides mainly existed in starch form. While the spectrum of ethanol extract was more clearly showing the correlation peaks of lipids and aromatics, and the number of peak and the shape of peak compared with oil standard, which has high overlap.[Conclusion] Dendrobium nobile contains polysaccharides and fatty substances. Polysaccharides mainly contain starch, and the content of fat in Dendrobium nobile is higher. Different extraction solvents appear in the spectrum there is a big difference, the perfect interpretation of the "similar compatibility" principle. Infrared spectroscopy provides a good method for the overall structure analysis and identification of Dendrobium nobile and quality control. |
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